pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter
(Plasmid #172596)

• Purpose: Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.
• Depositing Lab: Daniel Kiss
• Publication: Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 10.1093/hmg/ddac037.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10375
• Vector type: Mammalian Expression ; EGFP-PEST_Reporter
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myc-PDGFRA-R914W
• Alt name: Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
• Alt name: PDGFRA
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3324
• Mutation: PDGFRA-R914W
• Entrez Gene: PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Extracellular c-myc epitope tag (N terminal on insert)
  • Signal peptide from influenza HA (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pGEX 3'

Resource Information

• Supplemental Documents:
  • pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter.gb
• A portion of this plasmid was derived from a plasmid made by: Erik Procko (Addgene plasmid # 136456)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry