pcDNA3-mRuby2-MACROD1_d77
(Plasmid #172588)

• Purpose: For transient expression of C-terminal mRuby2-tagged MACROD1del1-77
• Depositing Lab: Karla Feijs
• Publication: Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y.

Backbone

• Vector backbone: pcDNA3-mRuby2
• Backbone size w/o insert (bp): 6140
• Total vector size (bp): 6888
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MACROD1
• Alt name: LRP16
• Species: H. sapiens (human)
• Insert Size (bp): 750
• Mutation: deleted amino acids 1-76
• Entrez Gene: MACROD1 (a.k.a. LRP16)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRuby2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry