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pcDNA3.1-MCS-BirA(R118G)-HA-TARG1
(Plasmid #172583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-MCS-BirA(R118G)-HA
  • Total vector size (bp) 6837
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TARG1
  • Alt name
    OARD1
  • Alt name
    C6orf130
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    453
  • Entrez Gene
    OARD1 (a.k.a. C6orf130, TARG1, dJ34B21.3)
  • Tag / Fusion Protein
    • BirA(R118G)-HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV fw
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-MCS-BirA(R118G)-HA-TARG1 was a gift from Karla Feijs (Addgene plasmid # 172583 ; http://n2t.net/addgene:172583 ; RRID:Addgene_172583)

  • For your References section:

    Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome. Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs KLH. Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y. 10.1038/s41598-020-64623-y PubMed 32427867
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