pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato
(Plasmid #172535)

• Purpose: Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance
• Depositing Lab: Richard White
• Publication: Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi: 10.7554/eLife.64744.

Backbone

• Vector backbone: pDestTol2pA2-Blasticidin
• Backbone size w/o insert (bp): 7011
• Total vector size (bp): 11934
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: -3.5ubb:plin2-tdtomato
• Species: D. rerio (zebrafish)
• Insert Size (bp): 6297
• Mutation: S80R, A81S, E144D, I148F and R415H mutations observed in PLIN2-tdtomato.
• Promoter: zebrafish ubb

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 Rev
• 3′ sequencing primer: M13 Fwd

Resource Information

• Supplemental Documents:
  • pDestTol2pA2-B.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function.