pME-PLIN2-tdTOMATO
(Plasmid
#172533)
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PurposePLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepME-tdTOMATO
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameplin2-tdtomato
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)2763
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MutationS80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-PLIN2-tdTOMATO was a gift from Richard White (Addgene plasmid # 172533 ; http://n2t.net/addgene:172533 ; RRID:Addgene_172533) -
For your References section:
An in vivo reporter for tracking lipid droplet dynamics in transparent zebrafish. Lumaquin D, Johns E, Montal E, Weiss JM, Ola D, Abuhashem A, White RM. Elife. 2021 Jun 11;10. pii: 64744. doi: 10.7554/eLife.64744. 10.7554/eLife.64744 PubMed 34114952
Map uploaded by the depositor.
