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Addgene

pcryGFP-acta1-dmdGFP
(Plasmid #172530)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172530 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDestTol2pACryGFP
  • Backbone manufacturer
    Joachim Berger
  • Backbone size w/o insert (bp) 7488
  • Total vector size (bp) 21551
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dmd
  • Alt name
    dystrophin
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    11466
  • Entrez Gene
    dmd (a.k.a. Dp7, Dp71, cb664, im:691178, im:6911785, sa, zfDY, zfDYS, zgc:110165)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcryGFP-acta1-dmdGFP was a gift from Joachim Berger (Addgene plasmid # 172530 ; http://n2t.net/addgene:172530 ; RRID:Addgene_172530)
  • For your References section:

    Effect of Ataluren on dystrophin mutations. Berger J, Li M, Berger S, Meilak M, Rientjes J, Currie PD. J Cell Mol Med. 2020 Jun;24(12):6680-6689. doi: 10.1111/jcmm.15319. Epub 2020 Apr 28. 10.1111/jcmm.15319 PubMed 32343037