pcryGFP-acta1-dmdGFP
(Plasmid
#172530)
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PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2pACryGFP
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Backbone manufacturerJoachim Berger
- Backbone size w/o insert (bp) 7488
- Total vector size (bp) 21551
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedmd
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Alt namedystrophin
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)11466
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Entrez Genedmd (a.k.a. Dp7, Dp71, cb664, im:691178, im:6911785, sa, zfDY, zfDYS, zgc:110165)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcryGFP-acta1-dmdGFP was a gift from Joachim Berger (Addgene plasmid # 172530 ; http://n2t.net/addgene:172530 ; RRID:Addgene_172530) -
For your References section:
Effect of Ataluren on dystrophin mutations. Berger J, Li M, Berger S, Meilak M, Rientjes J, Currie PD. J Cell Mol Med. 2020 Jun;24(12):6680-6689. doi: 10.1111/jcmm.15319. Epub 2020 Apr 28. 10.1111/jcmm.15319 PubMed 32343037