pcry:GFP,-503unc:hACTA1GFP
(Plasmid
#172529)
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PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDESTtol2pACrymCherry
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Backbone manufacturerJoachim Berger
- Backbone size w/o insert (bp) 6355
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATCA1
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SpeciesH. sapiens (human)
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Insert Size (bp)1134
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GenBank IDNM_001100.4
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcry:GFP,-503unc:hACTA1GFP was a gift from Joachim Berger (Addgene plasmid # 172529 ; http://n2t.net/addgene:172529 ; RRID:Addgene_172529) -
For your References section:
In Vivo Function of the Chaperonin TRiC in alpha-Actin Folding during Sarcomere Assembly. Berger J, Berger S, Li M, Jacoby AS, Arner A, Bavi N, Stewart AG, Currie PD. Cell Rep. 2018 Jan 9;22(2):313-322. doi: 10.1016/j.celrep.2017.12.069. 10.1016/j.celrep.2017.12.069 PubMed 29320728
