pcry:mCherry,-600unc:cct3GFP
(Plasmid
#172528)
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PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDESTtol2pACrymCherry
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Backbone manufacturerJoachim Berger
- Backbone size w/o insert (bp) 6353
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecct3-GFP
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SpeciesD. rerio (zebrafish)
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GenBank IDENSDARG00000016173
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcry:mCherry,-600unc:cct3GFP was a gift from Joachim Berger (Addgene plasmid # 172528 ; http://n2t.net/addgene:172528 ; RRID:Addgene_172528) -
For your References section:
In Vivo Function of the Chaperonin TRiC in alpha-Actin Folding during Sarcomere Assembly. Berger J, Berger S, Li M, Jacoby AS, Arner A, Bavi N, Stewart AG, Currie PD. Cell Rep. 2018 Jan 9;22(2):313-322. doi: 10.1016/j.celrep.2017.12.069. 10.1016/j.celrep.2017.12.069 PubMed 29320728