pcryBFP_Umyo18bGFP
(Plasmid
#172527)
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PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2pACryGFP
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Backbone manufacturerJoachim Berger
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyo18b
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)6309
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GenBank IDKX485371
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcryBFP_Umyo18bGFP was a gift from Joachim Berger (Addgene plasmid # 172527 ; http://n2t.net/addgene:172527 ; RRID:Addgene_172527) -
For your References section:
Myo18b is essential for sarcomere assembly in fast skeletal muscle. Berger J, Berger S, Li M, Currie PD. Hum Mol Genet. 2017 Mar 15;26(6):1146-1156. doi: 10.1093/hmg/ddx025. 10.1093/hmg/ddx025 PubMed 28104788