Skip to main content
Addgene

50_p5E_UBI_loxTagBFP
(Plasmid #172518)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p5E
  • Total vector size (bp) 7484
  • Modifications to backbone
    Insertion of a Ubiquitin promoter, a tagBFP gene flanked by LoxP site and 3x PolyA sequences

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin promoter, TagBFP, loxP sites (inversion)
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3497
  • Promoter Ubiquitin

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gtaaaacgacggccag
  • 3′ sequencing primer gtcatagctgtttcctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    50_p5E_UBI_loxTagBFP was a gift from Jean Giacomotto (Addgene plasmid # 172518 ; http://n2t.net/addgene:172518 ; RRID:Addgene_172518)

  • For your References section:

    Cre-Lox miRNA-delivery technology optimized for inducible microRNA and gene-silencing studies in zebrafish. Guo G, Tromp A, Wang H, Hall TE, Giacomotto J. bioRxiv 2024.02.22.581357 10.1101/2024.02.22.581357
Related items: