pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
(Plasmid
#172514)
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PurposeEncodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
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gRNA/shRNA sequenceUnspecified
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SpeciesDesulfonema ishimotonii
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 172514 ; http://n2t.net/addgene:172514 ; RRID:Addgene_172514) -
For your References section:
Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 10.1038/s41586-021-03886-5. 10.1038/s41586-021-03886-5 PubMed 34489594
