pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides
(Plasmid
#172506)
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PurposeEncodes DisCas7-11 protein alone with a golden gate site for spacer cloning
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnspecified
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDisCas7-11 bacterial expression vector with golden gate site for guides
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gRNA/shRNA sequenceUnspecified
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SpeciesDesulfonema ishimotonii
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 172506 ; http://n2t.net/addgene:172506 ; RRID:Addgene_172506) -
For your References section:
Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 10.1038/s41586-021-03886-5. 10.1038/s41586-021-03886-5 PubMed 34489594
