pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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GenBank

SnapGene

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> Addgene NGS Result AGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTC AAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCGTG CGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCA CGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGT CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAGCACCACT GGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCGCCGGTTAAGGCTAAACTGA AAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGA ACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAAC GATCTCAAGAAGATCATCTTATTAATCAGATAAAATATTTCTAGTTGATGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCGGTTGGAAAGCCGGTTTTCTTTGATGTCACGGAACCGAGACCTACTGTTGGTCTCGGGTTG GAAAGCCGGTTTTCTTTGATGTCACGGTTGCCACCCGGATCCCATGTGCGCTGTACCGGTGGTTCTCCGA CTGATCAAAGACCGGGCAGCCCCGGATGAATTTTACATCCGGGGCTGTTCCGCAGGCAAAATGCCAGAGA ATTCTGAGCACCGGAATCTGACGACATAACAAAAGGCAAAGTTGACTGAAAGAACAGGAACATTATCTGA AATTTTTACAAAAAGGAGGCAGATATGGTGACACCCGGCATGATTCAGAGCGCAGGCAAAGAGATGGGAC TTTTCATCCCGTGGGATGTGGCATTGGGCATGGTTCTCTGCGTTGCCGGAAAAATACTCATGGGCGAGAG CCTGGAAAATGCGGTTTTGTCGTGTTGGAAATAAAGCCCATTTTAAAATGTCGCGGATAAGATGAATAAT ACCAAATCGCAGATGGAATATGTCAGCCGGAGCAAGGCTCCGGCAATCCGCACTCCGAACAAAAGATAAA TTCAATACAGAATCGGTAGAAGCAGGGATTTTTTTTATTGACGCTGATAACAGGAAAAAGCTACAAACGT AGTTTGCAGATTTTTATCATTACCTACAACTTTTTTTAAAATCAGGATATTCGAAAATTATGAGTTTAAA TCATCCCTGTTTGTCTGCCCGTGCTGCGTCACCCGAATGTACTGTTTGTCCTGTTTTTAAGGCTGGGCAT CAGGAGGCATTTGTCAAAATTCTTGAAAAAAAGGTTCGGTTTCTCGAAAGCCGAAAACATCTGTCACCGG AAGACAAGGAAGATATTCTCAGCGAATTTGTTCTGCAAATCCAGTCTTCCACATCCTCATTTAAGGGACA GAATGCAAAATTTGAAACCTGGGCCTATAGAATATTTCAAAATGTATGGGCTGATTTTTTTAGAAAAATC AAAAATTTCAAAACGCAGAGAACCGATGACGGCATTTATATTCAGGAAACAATAAAAGTAAAAAAAACGG ATAAAGACGGAAGAGAAATGCTTGAGGAAAAAGCAAGGACGGGCGTATTTCTTCCATATAAAGAGAATAT CGAAGACAGAATTGAATCGCGGGAAATTTTTTCAATTTTCCGCAAAGCAGTAAAAGATCCGAAAAAGGCA TTTTGCTATCATTTGCTTGTTAATCATTATACTTGGAAAAGCGAAGGGTTGAAACAGGATGAAATGGCGG CTCGGTATGAAATGAGGCACGATACCTTCAGAAAACGCCTTTCAGAATGCCGCTCAAGATTCAAACAATA TCTGAAAGAGGTTACGCAATGACAATTCCCGAAATCTGCCGCGAGCCAGGATATGTTTTTTACGAAGGAA TTTTTGAAAGTGTTCTTTCGCGTGACTGTTATTGCTTTAAAGTGGAGAACGAGGATTACAGCGGAGAGGT GATAATGGAAAAACATTATTCCCCCGACAATGTGCAGCTAAAAAGCGGTAAACCTGTCACTGACCTTGTT GTTGTTAGATATGAGAAAAACCGTCTGTTTGTGGCCATGAATAATTATTTTGATGATCTGATGGGGCATT TTTTCAATACGGACAGAGGAGTTTTTGTAAAAAATATTCCTTTTCCAAACTGGGAAAGGGCTTGGGAAAC AGCGTTTGCCCATACAAATTATCAGCCTGAAAAGTTGCAACTCAAGAAGAGAGAACACAGAAACGAACGA TGGATTGAGGTAAAAAGTCCCCGGTGTCGGATACCTGCCCATGAACATTCTCATATCAGTGATATGGACG GGGAACCAGTGTTTTTTCCTGCCTCATTTTACAATATACAAAACAGAGACAGGAACGGCAGGTTGATTCC CCGCGCACTGTATAAAACCGAAGGCATGGAGGAGATGCCCTGTTACTTCAAAGGGGAATTCAGCCCGGAA TCCGGCAACACCCGGACAGTCCACATTTATCTGCATGGCATAACGTTTCCTTCCAAATGGCATTATAGCG TATTGAAGCACATGATCTGTCTTCCGAAATTCAAAGGCAAGGATGACAATCGCGACCTGTGGAAAGCATT TGATGGAGAAGAACCGCTTGAACCAGACGTAGAAATTGTGCAAGCGTGTAAACTGAAATGCAAAGATTCA TGTGATTAAAGGCATTGCCAAAAGGAAAAATTTTCAAGAATGTTCACAAAAAGATCAGAACAGCGTGTGA ACGGTATCTGAGACGGGCCATCATATTTTTTCCCCAACATACCGACAAAGCTCTGAGGATTTGGCAAGAC AGGAAGAGCCAGTGCGGAAAACCCAAATTTATTGAGGTGGATTGTCTGAATAAAATCGAAAACAACATAT TCAGACAAGCTCTCGGGCAGGAAGAGTTGGAGCGGAATGAAATTCTCATCCTCCGGGGATATTCATATGA CTGGGATTCCGCCGAGGAACCAACGATTCATCTGAGTATTGTCACAATGTATGCAGGCCCCTTAAAAGGG AAAATGCCCCGTGAAAACGCATTCAAAAAAGATTTCAGGCTGACCGGAAACGGCAAACTTTCCATATCGG AGAATTAAGAGACAAAATGAATACAACAACATACAACACCACAACAGACGCGCTTTTGGAATGGGGCAAG GTATATTTTCAGAAAGAGGATTTTTCTGAATTCCTTGATAATCTCGAAGCGTATATTTCAGATGCCGGGG ACAGTCTGAAAGATGAACTTGAGAGCGGGGTGGAAAAGCTTGTTTTGGGCATAAAATCGGCGGAAGCAGT TATTTTCGGAGAGGCTGTGATCGGGACTACGCCGGAAAATGAAGCATGGTACGATGCCGAAGAATCCTTT CTGACTTTGGATTGTGCCGTATGGCTGTCCCAAGCGCTTGATCGCGTTGTTCGCAGGCAGGATGCGTCAT TGGCCGACAGCCTGATCGCCCGCTTGGACGAAGCCATAAACCGCGTCGCCGAAAAACTGTATGCGGACAA TTTATCTCCGCTTCGTTTTTCATCGCTCAATGAAATCCGTCGAAGCGCTCTGGAGGCAACGGATGAGAAA TATCATTATCTGTTTCCCTGGCACGGGGCCGCATGTGATGTTGATGAAAACATTCTGCTGATTCTGACGG AGGAATATCATCTGATCGGGGCAGATAAAGCCAGAGCCAATCTTTCAGAGGAACTGCGGGGCGACCTGCC TTTTATTTTTGCCGAGTTGGAGCGGGATGAGGTGCTGCGGGCATATGTTGAAAAGGAGAACGCCCTTTCT CTTGCTTTGGAAAATACCATGCGGGAACACTGGGCATTCGGCCTGCTGGAAGCGGCCCGTGATGAAGGGT ACAACCATCCCTATCCCGCAGATGTCGGGATGAGGATTCATCAGGTTGCCCGTGCCGTATTTTCCCAGAC AAACCTCTCTCCGGCGGAACGCCTTGCTGTTGCCATAGCCGGGGCCTGTTTTACGCCTGAGATCAGCGAA GACCGGCGGCTTGAGATTCTGCTGGACTGTGAGGAGCGGGTGTGTGAAATCGAAGCCCCGACCGGAGACG ATACATCCGTCCGCGTTATTAAAGACTTGAAAGCGCTGGCCAATCACCGTGTCCGCCATGAAATCCCGGC AGAAAGTCTTGTCAGTCTGTGGTTTGAGCAGATTGAGGCGGCGGGGACGGATTTTGATACAAAGACACCG ATGGATGAATTGGTGTTGCGAATGCTTTCCGATAACGTCATCACTCTGTCTGTTGACCGAAAAGCGGCTT CTCAGACAGAAACAGATGATGTGAAACCGCAGAAAGGAAAAATCATACCCTTTCCTGTTCCTGATATTGC CAACGATGAAGTTGAATACCAAAAAGCTGTGGGAATGAAGAAGGATAAAAAGGCGACTAATGACAGCAAA GTCAAATTTCCCGGCCTGCTTGAAATCCAGGGTTGTCGTGACGGGGACAAGGCTATTTTGCTGGAAGACA CAGACGATGCGGCAGCTAATCACCGGAAACTGTTTTCCATTCTGAAAGCAGGTAAATTGAATTCAGCTTT TTTCATTCAAAGCGATGATGGAGAATGGGTTGAGTCGGAATCCAAACCGACGATGGAAGATAACCGTATC ATATTACATGACAGCCATCATTCCAGCTTTGTATGGATATTGGATACCGGTTCCATGCAGCTCAGACAGA GTGTAAAGTGTGTCAAAGACGCATTGAATAAGAAGACAGGGAGTGCGAAGAAACTGAAACCGAAAACAAT GATCGTTTGGGTCACGATTCCGCAGGAGGGATGATATGTCAAATCCGATTCGGGATATTCAGGACAGGCT GAAAACTGCAAAATTTGACAATAAGGATGATATGATGAACTTGGCATCCTCGTTGTACAAATATGAAAAG CAACTGATGGATTCTTCTGAAGCAACACTTTGTCAGCAAGGGTTGTCCAATCGGCCCAATTCATTCAGCC AGCTTTCCCAATTCCGGGATTCCGACATTCAAAGTAAAGCAGGCGGACAGACGGGGAAATTTTGGCAGAA TGAATATGAAGCCTGCAAAAATTTTCAAACCCATAAAGAGCGGCGGGAAACTCTGGAGCAAATCATCCGC TTTCTCCAAAATGGCGCAGAAGAAAAAGATGCGGATGATCTGCTTCTGAAAACGCTTGCCCGCGCCTATT TTCACAGGGGGTTGCTCTACAGGCCCAAAGGCTTCTCTGTTCCGGCCAGGAAAGTCGAAGCAATGAAAAA GGCAATAGCGTACTGTGAAATAATTCTGGACAAAAATGAAGAAGAGTCGGAGGCACTAAGAATCTGGCTT TATGCCGCAATGGAACTCCGACGGTGCGGTGAGGAGTATCCGGAAAATTTCGCTGAAAAACTTTTTTATC TCGCAAATGACGGTTTCATATCCGAACTGTATGATATCAGACTTTTTCTTGAATATACGGAGCGAGAAGA AGATAACAATTTTCTTGATATGATCCTTCAGGAAAATCAGGACAGGGAACGCCTTTTTGAACTATGTCTT TACAAGGCCAGAGCGTGTTTCCATCTAAATCAGTTGAATGACGTGAGAATATATGGAGAAAGTGCAATTG ATAACGCGCCGGGGGCTTTTGCCGATCCGTTCTGGGATGAGCTTGTTGAATTTATCAGAATGCTTAGAAA TAAGAAATCGGAATTATGGAAAGAAATTGCCATAAAAGCGTGGGACAAGTGCCGCGAAAAAGAAATGAAA GTTGGCAACAATATTTATCTAAGCTGGTATTGGGCCAGACAGCGAGAGCTTTATGACCTTGCGTTTATGG CGCAGGACGGTATTGAAAAAAAAACGAGAATTGCTGATTCCCTGAAATCCAGAACCACACTCAGAATCCA GGAATTGAATGAACTCAGAAAAGATGCACATAGAAAACAAAATAGGAGATTAGAAGACAAACTGGATAGG ATTATTGAGCAGGAAAATGAAGCCAGAGATGGTGCTTATCTAAGGAGAAATCCTCCTTGCTTTACAGGCG GAAAAAGGGAAGAAATACCTTTTGCCAGACTGCCCCAAAACTGGATTGCCGTCCATTTCTATCTGAACGA ACTGGAATCTCACGAAGGCGGAAAAGGCGGACATGCGCTGATTTATGATCCTCAAAAAGCTGAAAAAGAT CAATGGCAGGACAAATCCTTTGACTACAAAGAACTGCATCGGAAATTTTTGGAGTGGCAGGAAAACTATA TTCTGAACGAAGAAGGGTCCGCCGATTTTCTGGTAACACTCTGTCGGGAAATCGAAAAAGCCATGCCTTT TCTGTTTAAGTCAGAAGTGATTCCCGAAGACAGGCCGGTATTGTGGATTCCGCATGGTTTCCTGCATCGA CTCCCCCTTCATGCAGCCATGAAATCAGGTAATAACTCCAATATTGAAATATTTTGGGAAAGACATGCGT CTCGATATCTTCCTGCATGGCATCTGTTTGATCCTGCTCCATATAGTAGAGAAGAAAGCTCCACTCTTTT AAAGAATTTTGAAGAATATGATTTCCAGAATTTGGAAAATGGAGAAATTGAGGTTTACGCCCCCTCATCT CCCAAGAAAGTAAAAGAGGCAATTAGGGAAAATCCGGCCATTCTGCTTTTGCTTTGTCATGGTGAAGCCG ACATGACCAATCCTTTTCGCTCCTGCCTGAAGCTTAAAAATAAAGATATGACCATCTTTGATCTGCTGAC AGTGGAAGATGTAAGGCTCTCCGGTTCACGAATTCTTCTGGGTGCCTGTGAGTCCGATATGGTTCCACCG TTGGAGTTTTCTGTGGACGAACACCTTTCTGTTAGCGGTGCATTTCTTTCTCACAAGGCGGGAGAGATTG TTGCGGGTCTATGGACAGTTGATTCGGAAAAAGTTGACGAATGCTATTCGTATTTGGTGGAGGAGAAAGA TTTTTTGAGAAATTTGCAGGAATGGCAAATGGCAGAAACAGAAAACTTTCGGTCTGAAAATGATAGTTCT TTATTTTATAAAATCGCCCCGTTCAGAATCATCGGCTTCCCGGCGGAATGATATAACCCACACGTCTTCC TTCTTTCCCGATCCAGCAGAGCTACAGGGAAAGCGTATAAGAACGGGAAAAATTCAGGCCGGAGCGGGAC TTTCCTTTTCTCACACATTTTCCCTGTAGTTTTCCCAAGGCTTTGAGGGACGATTTCCACGCGACATACC GAGGAGACACATACCATGACGACGACTATGAAAATTTCCATCGAATTTCTCGAACCCTTTCGGATGACTA AATGGCAGGAAAGTACCAGACGAAACAAAAACAACAAAGAATTCGTCCGGGGTCAGGCATTTGCCAGATG GCACAGAAACAAAAAAGACAACACAAAGGGCCGTCCCTATATCACCGGAACCCTGCTCCGTTCGGCAGTC ATCCGCTCGGCGGAGAACCTGCTGACGCTTTCCGATGGAAAAATTTCAGAAAAGACCTGTTGTCCGGGGA AATTCGACACTGAAGACAAAGACAGATTGTTACAATTGCGCCAGCGTTCCACGCTCAGGTGGACAGATAA AAATCCCTGCCCGGACAACGCCGAAACATATTGCCCGTTCTGTGAACTGCTGGGCCGATCCGGTAACGAC GGAAAGAAAGCCGAAAAAAAAGATTGGAGATTTCGTATCCATTTCGGCAATCTCAGTTTGCCTGGAAAAC CGGACTTTGACGGCCCGAAGGCAATCGGATCACAGCGCGTTCTGAACCGGGTCGATTTCAAAAGCGGCAA GGCCCATGACTTTTTTAAAGCCTATGAAGTGGATCATACCCGCTTTCCCCGGTTTGAGGGAGAGATCACC ATTGACAACAAGGTGAGTGCCGAGGCCCGAAAGCTTCTGTGCGACAGCCTGAAATTCACAGACCGGCTCT GCGGGGCCTTGTGTGTCATCCGGTTTGACGAGTATACTCCGGCTGCCGATTCCGGCAAACAGACGGAGAA TGTGCAGGCAGAGCCAAATGCCAATCTCGCCGAGAAAACCGCCGAGCAGATCATCAGCATCTTGGATGAC AACAAAAAAACCGAATACACCCGCCTTCTGGCTGATGCCATCCGTTCGCTCCGCCGGAGCAGCAAGCTTG TCGCCGGTCTGCCCAAAGATCACGATGGAAAGGATGATCATTATCTCTGGGACATCGGAAAGAAAAAAAA AGATGAAAACAGCGTAACAATCCGCCAAATCCTGACAACATCGGCGGATACAAAAGAACTTAAAAATGCC GGAAAGTGGCGCGAATTCTGCGAGAAGCTGGGCGAGGCACTCTACCTGAAATCCAAAGACATGAGCGGCG GCTTGAAAATAACCCGGCGAATTCTGGGAGACGCCGAATTTCACGGAAAGCCGGATCGTCTGGAAAAATC CCGTTCTGTTTCCATCGGCTCTGTGCTGAAAGAGACGGTTGTCTGCGGAGAACTGGTCGCAAAAACGCCG TTTTTCTTCGGAGCCATTGATGAGGATGCAAAACAGACCGATCTTCAGGTGTTGCTGACGCCCGACAACA AATACCGCCTGCCGCGCTCCGCTGTTCGGGGAATCCTGCGCCGGGATTTGCAGACATACTTTGACAGCCC GTGCAATGCCGAACTCGGCGGAAGGCCCTGTATGTGCAAAACCTGCCGGATCATGCGCGGTATCACCGTC ATGGATGCCCGCAGTGAATACAATGCGCCGCCGGAAATCCGCCACCGCACCCGCATCAATCCCTTTACCG GCACGGTTGCCGAGGGAGCGCTGTTCAATATGGAAGTGGCACCGGAGGGAATTGTGTTTCCCTTCCAGCT TCGCTACCGGGGAAGCGAAGACGGATTGCCGGATGCACTGAAAACCGTCCTGAAATGGTGGGCAGAGGGC CAGGCGTTTATGAGCGGCGCGGCATCCACGGGGAAAGGGCGATTCCGTATGGAAAACGCCAAGTATGAGA CATTGGATTTGTCCGATGAAAATCAACGGAATGATTATCTGAAAAATTGGGGATGGCGGGATGAAAAAGG ATTGGAGGAACTGAAAAAACGCCTGAATTCTGGCTTGCCGGAACCGGGCAATTACCGTGACCCCAAGTGG CACGAAATCAACGTCAGTATAGAAATGGCCTCCCCCTTCATCAATGGCGACCCGATCCGCGCCGCCGTGG ATAAAAGGGGTACGGATGTGGTCACGTTCGTCAAATACAAAGCCGAAGGTGAAGAGGCCAAACCAGTTTG TGCCTACAAGGCGGAGAGTTTCAGGGGCGTGATACGTTCGGCAGTTGCGCGAATCCATATGGAAGATGGT GTTCCGCTGACCGAACTGACCCACAGCGATTGCGAATGCCTGCTCTGCCAGATTTTCGGCAGCGAATATG AGGCCGGGAAAATCCGGTTTGAGGATCTGGTGTTTGAATCAGATCCAGAGCCGGTGACATTCGACCATGT GGCCATTGACCGGTTTACCGGCGGCGCGGCTGACAAAAAGAAATTTGACGATTCTCCGCTTCCGGGCAGT CCGGCCAGACCTCTCATGCTAAAAGGCAGCTTCTGGATACGGCGGGATGTTCTGGAAGATGAGGAATATT GCAAAGCGCTGGGAAAGGCGCTGGCAGATGTGAACAACGGGCTTTATCCTCTGGGCGGAAAAAGCGCCAT CGGATATGGGCAGGTAAAAAGTCTGGGGATTAAAGGAGATGATAAGCGGATTTCCCGTTTGATGAACCCC GCTTTTGATGAAACAGATGTGGCTGTGCCGGAAAAGCCGAAAACCGATGCCGAGGTTCGGATTGAGGCGG AAAAGGTTTATTATCCTCATTATTTTGTCGAGCCTCATAAAAAGGTGGAGCGTGAAGAAAAGCCTTGCGG CCATCAGAAATTTCATGAAGGACGGCTGACCGGAAAAATTCGATGCAAACTGATCACAAAGACGCCGCTC ATTGTACCGGACACCTCGAATGACGATTTTTTCAGGCCCGCAGACAAAGAAGCCAGAAAAGAAAAAGATG AGTATCACAAAAGCTATGCCTTTTTCAGGCTTCACAAGCAAATTATGATTCCCGGTTCCGAGCTGCGGGG CATGGTCAGTTCGGTTTACGAAACCGTGACCAACTCCTGCTTTCGCATTTTTGATGAAACCAAGCGGCTT TCGTGGCGGATGGATGCAGACCATCAGAATGTGCTTCAAGATTTTCTTCCCGGCAGAGTTACGGCTGACG GAAAGCATATACAAAAATTTTCTGAAACCGCACGGGTTCCTTTTTATGATAAAACCCAGAAGCATTTTGA CATATTGGATGAACAGGAAATAGCAGGCGAAAAACCTGTTAGAATGTGGGTCAAAAGATTTATCAAACGT TTGTCTCTGGTCGATCCTGCTAAACATCCTCAAAAAAAGCAGGACAACAAATGGAAGCGCAGAAAAGAGG GAATAGCCACATTCATAGAACAGAAAAACGGCAGTTATTATTTCAATGTAGTTACGAACAATGGCTGTAC GAGCTTTCATTTATGGCACAAACCTGACAACTTTGACCAAGAGAAACTTGAAGGTATCCAGAATGGTGAG AAATTGGATTGTTGGGTGAGAGATTCCAGATATCAAAAGGCTTTCCAGGAAATACCCGAAAATGATCCGG ATGGATGGGAATGTAAAGAAGGATACCTTCATGTTGTCGGTCCCAGCAAAGTAGAGTTTTCCGACAAGAA AGGTGATGTCATAAACAATTTTCAGGGAACCCTGCCATCTGTACCGAATGATTGGAAAACGATCCGAACC AATGATTTCAAAAACCGAAAACGCAAAAATGAACCCGTATTTTGCTGTGAGGATGATAAGGGAAACTATT ATACGATGGCAAAATATTGTGAAACCTTTTTCTTTGACCTCAAAGAAAATGAGGAGTATGAAATTCCAGA AAAAGCCCGTATTAAATACAAAGAGCTGTTGAGAGTTTATAACAATAATCCCCAAGCAGTTCCTGAATCG GTATTTCAATCTCGCGTCGCAAGAGAAAATGTCGAAAAGCTTAAATCTGGCGATCTTGTCTATTTTAAAC ACAATGAAAAATATGTTGAGGATATTGTTCCCGTCCGCATTTCCCGGACAGTTGATGATCGGATGATCGG CAAACGCATGTCAGCCGATCTCCGCCCCTGTCACGGTGACTGGGTGGAAGACGGCGACCTTTCCGCCCTG AACGCCTACCCGGAAAAGCGCCTTCTCCTGCGTCATCCCAAAGGATTGTGCCCGGCCTGCCGCCTTTTCG GAACCGGTTCATACAAGGGCCGGGTTCGGTTCGGATTTGCAAGCCTTGAAAATGATCCCGAATGGCTGAT ACCCGGCAAGAATCCCGGTGATCCCTTCCACGGGGGACCGGTAATGCTGTCGCTTCTGGAGCGCCCCCGG CCCACATGGTCGATCCCCGGATCGGACAATAAGTTCAAAGTGCCGGGCCGGAAGTTTTATGTCCATCATC ATGCATGGAAAACGATAAAAGACGGCAACCATCCCACAACCGGAAAGGCCATAGAACAAAGCCCCAACAA CCGGACAGTTGAAGCCTTGGCCGGGGGAAACAGCTTTTCTTTTGAAATCGCTTTTGAAAATCTGAAAGAA TGGGAACTCGGTTTGCTGATTCATTCCCTGCAATTGGAAAAAGGGTTGGCACACAAGCTGGGCATGGCAA AATCAATGGGATTCGGCAGCGTTGAGATTGATGTTGAATCTGTGCGTCTGAGGAAGGACTGGAAACAGTG GAGAAACGGAAACAGTGAAATACCAAACTGGCTTGGGAAAGGCTTTGCCAAGCTAAAGGAATGGTTCAGA GATGAGCTGGATTTTATAGAAAATCTGAAGAAGTTGCTCTGGTTTCCAGAAGGCGACCAAGCGCCGAGAG TCTGTTATCCGATGCTGAGAAAAAAGGATGACCCGAACGGAAACTCCGGCTACGAAGAACTGAAAGACGG CGAATTTAAAAAGGAAGACCGCCAGAAAAAGCTCACCACGCCTTGGACCCCTTGGGCCTGATCTTCTGTG CCGCCTGATACTCTCCCTTCGTAAGATGATTCAGCATGAAAAGTTCATTATTTCAGCCCGGAAGTTCATT TTCGGGCTGAAACCTGTTGGTTTCCTCCGATAACCCGCTTCAGCGGGTTTCAATAACTGAGCCGGACCGG GTCGAATTTGCTTTCGAATTTCTGCCATTCATCCGCTTATTATCACTTATTCAGGCGTAGCAACCAGGCG TTTAAGGGCACCAATAACTGCCTTAAAAAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAA TTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATC AGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGG CCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGACACGAAAAACATATTCTCAATAAA CCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGC CGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAAC AAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATT CATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAA AAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAAT GTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTC CTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAG TTGGAACCTCTTACGTGCCGATCAACGTGTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAA CAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCGCAAAGTGC GTCGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGT TTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACAT CAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGT GGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGC TCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTT CCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTG

pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer Sequences (1)

Full Sequences from Addgene (1)

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