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Addgene

pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
(Plasmid #172501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unspecified
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DisCas7-11 full locus with Golden gate acceptor site for spacer
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Desulfonema ishimotonii

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 172501 ; http://n2t.net/addgene:172501 ; RRID:Addgene_172501)

  • For your References section:

    Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 10.1038/s41586-021-03886-5. 10.1038/s41586-021-03886-5 PubMed 34489594
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