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Addgene

pCM1.53
(Plasmid #17250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR201
  • Backbone size w/o insert (bp) 2257
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-no stop in pDONR201
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    908

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP1 (destroyed during cloning)
  • 3′ cloning site attP2 (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM1.53 was a gift from Geraldine Seydoux (Addgene plasmid # 17250 ; http://n2t.net/addgene:17250 ; RRID:Addgene_17250)

  • For your References section:

    Transgenic solutions for the germline. Merritt C, Seydoux G. WormBook. 2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1. 10.1895/wormbook.1.148.1 PubMed 20169625
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