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pET_His6-SUMO-TEV-TtCsm6
(Plasmid #172486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Total vector size (bp) 7060
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T. thermophilus Csm6 (codon optimized)
  • Alt name
    TTHB152
  • Species
    Synthetic
  • Entrez Gene
    csm6 (a.k.a. TTH_RS10780, TTHB152)
  • Promoter T7 promoter and lac operator
  • Tag / Fusion Protein
    • His-SUMO-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer SUMO forward (5'-ttattgaggctcacagagaac-3')
  • 3′ sequencing primer T7 reverse (5'-GCTAGTTATTGCTCAGCGG-3')
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GenScript and QB3 Macrolab (UC Berkeley)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon-optimized TtCsm6 gene ordered as a synthetic gene from GenScript in pUC57. It was then subcloned into a bacterial expression vector by QB3 Macrolab at UC Berkeley.

Please visit https://www.medrxiv.org/content/10.1101/2021.03.19.21253328v1 for medRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_His6-SUMO-TEV-TtCsm6 was a gift from Jennifer Doudna (Addgene plasmid # 172486 ; http://n2t.net/addgene:172486 ; RRID:Addgene_172486)

  • For your References section:

    Accelerated RNA detection using tandem CRISPR nucleases. Liu TY, Knott GJ, Smock DCJ, Desmarais JJ, Son S, Bhuiya A, Jakhanwal S, Prywes N, Agrawal S, Diaz de Leon Derby M, Switz NA, Armstrong M, Harris AR, Charles EJ, Thornton BW, Fozouni P, Shu J, Stephens SI, Kumar GR, Zhao C, Mok A, Iavarone AT, Escajeda AM, McIntosh R, Kim S, Dugan EJ, Pollard KS, Tan MX, Ott M, Fletcher DA, Lareau LF, Hsu PD, Savage DF, Doudna JA. Nat Chem Biol. 2021 Sep;17(9):982-988. doi: 10.1038/s41589-021-00842-2. Epub 2021 Aug 5. 10.1038/s41589-021-00842-2 PubMed 34354262
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