pCS2 Bighead S-HA
(Plasmid
#172485)
-
Purposefor synthesis of mRNA for injection into xenopus laevis embryos
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 172485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCS2
-
Vector typein vitro mRNA synthesis
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameBighead.S
-
Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- S-HA
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS2 Bighead S-HA was a gift from Edward De Robertis (Addgene plasmid # 172485 ; http://n2t.net/addgene:172485 ; RRID:Addgene_172485) -
For your References section:
Bighead is a Wnt antagonist secreted by the Xenopus Spemann organizer that promotes Lrp6 endocytosis. Ding Y, Colozza G, Sosa EA, Moriyama Y, Rundle S, Salwinski L, De Robertis EM. Proc Natl Acad Sci U S A. 2018 Sep 25;115(39):E9135-E9144. doi: 10.1073/pnas.1812117115. Epub 2018 Sep 12. 10.1073/pnas.1812117115 PubMed 30209221