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pEVOL-pylT-AznLRS
(Plasmid #172482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVOL
  • Total vector size (bp) 5651
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AznLRS and pylTcua
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1365
  • Promoter araBAD

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATG CCA TAG CAT TTT TAT CC
  • 3′ sequencing primer GAT TTA ATC TGT ATC AGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-pylT-AznLRS was a gift from Wenshe Liu (Addgene plasmid # 172482 ; http://n2t.net/addgene:172482 ; RRID:Addgene_172482)

  • For your References section:

    A Versatile Approach for Site-Specific Lysine Acylation in Proteins. Wang ZA, Kurra Y, Wang X, Zeng Y, Lee YJ, Sharma V, Lin H, Dai SY, Liu WR. Angew Chem Int Ed Engl. 2017 Feb 1;56(6):1643-1647. doi: 10.1002/anie.201611415. Epub 2017 Jan 2. 10.1002/anie.201611415 PubMed 28042700
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