mito-meGFP
(Plasmid #172481)

• Purpose: meGFP targeted to the mitochondrial matrix with a human COX8 presequence
• Depositing Lab: Thomas Schwarz
• Publication: Fluorescent plasmids for live cell imaging in primary neurons (unpublished)

Backbone

• Vector backbone: addgene plasmid 55838
• Backbone manufacturer: addgene
• Backbone size w/o insert (bp): 3929
• Total vector size (bp): 4715
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mito
• Alt name: COX8A presequence
• Species: H. sapiens (human)
• Insert Size (bp): 75
• GenBank ID: NM_004074.3 NM_004074.3
• Promoter: CMV
• Tag / Fusion Protein:
  • meGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 55838 and 54767.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry