CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons
(Plasmid #172480)

• Purpose: H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene
• Depositing Lab: Mario Capecchi
• Publication: Fluorescent protein with synthetic intron plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-sfGFP-T2A-tdTomato
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** 8 Hipp11 CAG-loxP-3xpA-H2BsfGFP-syn-T2A GST-tdTom-syntrons-from sequence.gb
• A portion of this plasmid was derived from a plasmid made by: CAG promoter is from pCAGGS obtained from BCCM/LMBP. SV40 pA is from Clontech. HIST1H2BB is from plasmid #20972 (https://www.addgene.org/20972/). sfGFP is from Sandia Biotech. tdTomato is from Roger Tsien. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland. Backbone is from Stratagene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry