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Addgene

pCG150
(Plasmid #17247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDESTR4R3
  • Backbone size w/o insert (bp) 4115
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    Needs ccdB resistant strain.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    unc-119 rescue fragment in pDESTR4R3
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2237

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR4 (destroyed during cloning)
  • 3′ cloning site attR3 (destroyed during cloning)
  • 5′ sequencing primer M13Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCG150 was a gift from Geraldine Seydoux (Addgene plasmid # 17247 ; http://n2t.net/addgene:17247 ; RRID:Addgene_17247)

  • For your References section:

    3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt C, Rasoloson D, Ko D, Seydoux G. Curr Biol. 2008 Sep 23. ():. 10.1016/j.cub.2008.08.013 PubMed 18818082
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