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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePDONRP4P1R
- Backbone size w/o insert (bp) 2645
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Vector typeGateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepie-1 intron:pie-1 promoter in PDONRP4P1R
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SpeciesC. elegans (nematode)
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Insert Size (bp)3044
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attP4 (destroyed during cloning)
- 3′ cloning site attP1R (destroyed during cloning)
- 5′ sequencing primer M13Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCG142 was a gift from Geraldine Seydoux (Addgene plasmid # 17246 ; http://n2t.net/addgene:17246 ; RRID:Addgene_17246) -
For your References section:
3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt C, Rasoloson D, Ko D, Seydoux G. Curr Biol. 2008 Sep 23. ():. 10.1016/j.cub.2008.08.013 PubMed 18818082