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Addgene

pIRES-EGFP-TPD54
(Plasmid #172459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-EGFP-Puro
  • Backbone manufacturer
    Addgene 45567
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPD54
  • Alt name
    TPD52L2
  • Alt name
    Tumor Protein D52 protein like 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TPD52L2 (a.k.a. D54, TPD54)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-EGFP-TPD54 was a gift from Stephen Royle (Addgene plasmid # 172459 ; http://n2t.net/addgene:172459 ; RRID:Addgene_172459)

  • For your References section:

    Intracellular nanovesicles mediate alpha5beta1 integrin trafficking during cell migration. Larocque G, Moore DJ, Sittewelle M, Kuey C, Hetmanski JHR, La-Borde PJ, Wilson BJ, Clarke NI, Caswell PT, Royle SJ. J Cell Biol. 2021 Oct 4;220(10). pii: 212493. doi: 10.1083/jcb.202009028. Epub 2021 Jul 21. 10.1083/jcb.202009028 PubMed 34287617
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