mCherry-FKBP-TPD52
(Plasmid #172456)

• Purpose: Expression of Tumor Protein D52 (TPD52) with N-terminal mCherry-FKBP tag
• Depositing Lab: Stephen Royle
• Publication: Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 212493. doi: 10.1083/jcb.202009028. Epub 2021 Jul 21.

Backbone

• Vector backbone: pmCherry-C1
• Modifications to backbone: mCherry-FKBP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TPD52
• Alt name: TPD52
• Alt name: Tumor Protein D52
• Species: H. sapiens (human)
• GenBank ID: BC006804.1
• Entrez Gene: TPD52 (a.k.a. D52, N8L, PC-1, PrLZ, hD52)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry-FKBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • mCherry-FKBP-TPD52.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry