GFP-FKBP-TPD52
(Plasmid
#172455)
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PurposeExpression of Tumor Protein D52 (TPD52) with N-terminal GFP-FKBP tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Modifications to backboneEGFP-FKBP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTPD52
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Alt nameTPD52
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Alt nameTumor Protein D52
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SpeciesH. sapiens (human)
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GenBank IDBC006804.1
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Entrez GeneTPD52 (a.k.a. D52, N8L, PC-1, PrLZ, hD52)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP-FKBP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site MfeI (not destroyed)
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypCMV-Sport6-TPD52 (Open Biosystems clone MHS1010-58208)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-FKBP-TPD52 was a gift from Stephen Royle (Addgene plasmid # 172455 ; http://n2t.net/addgene:172455 ; RRID:Addgene_172455) -
For your References section:
Intracellular nanovesicles mediate alpha5beta1 integrin trafficking during cell migration. Larocque G, Moore DJ, Sittewelle M, Kuey C, Hetmanski JHR, La-Borde PJ, Wilson BJ, Clarke NI, Caswell PT, Royle SJ. J Cell Biol. 2021 Oct 4;220(10). pii: 212493. doi: 10.1083/jcb.202009028. Epub 2021 Jul 21. 10.1083/jcb.202009028 PubMed 34287617