FLAG-TPD54
(Plasmid #172450)

• Purpose: Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal FLAG tag
• Depositing Lab: Stephen Royle
• Publication: Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb.201812044. doi: 10.1083/jcb.201812044.

Backbone

• Vector backbone: pFLAG-C1
• Modifications to backbone: FLAG in place of EGFP or mCherry
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TPD54
• Alt name: TPD52L2
• Alt name: Tumor Protein D52 protein like 2
• Species: H. sapiens (human)
• GenBank ID: BC006804.1
• Entrez Gene: TPD52L2 (a.k.a. D54, TPD54)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • FLAG-TPD54.gb
• A portion of this plasmid was derived from a plasmid made by: pCMV-Sport6-TPD52L2 (Open Biosystems clone MHS1010-57728)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry