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Addgene

mCherry-BAF
(Plasmid #172447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAF
  • Alt name
    Barrier to autointegration factor-1
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid 101772

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-BAF was a gift from Stephen Royle (Addgene plasmid # 172447 ; http://n2t.net/addgene:172447 ; RRID:Addgene_172447)

  • For your References section:

    Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes. Ferrandiz N, Downie L, Starling GP, Royle SJ. J Cell Biol. 2022 Jun 6;221(6). pii: 213189. doi: 10.1083/jcb.202203021. Epub 2022 Apr 29. 10.1083/jcb.202203021 PubMed 35486148
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