LBR-mCherry
(Plasmid #172446)

• Purpose: Expression of nuclear envelope protein LBR with a C-terminal mCherry tag
• Depositing Lab: Stephen Royle
• Publication: Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213189. doi: 10.1083/jcb.202203021. Epub 2022 Apr 29.

Backbone

• Vector backbone: pmCherry-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: lamin B receptor
• Species: H. sapiens (human)
• Entrez Gene: LBR (a.k.a. C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • LBR-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene 646

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.23.441091v3.