Stargazin-dCherry-FRB
(Plasmid #172444)

• Purpose: Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB
• Depositing Lab: Stephen Royle
• Publication: Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213189. doi: 10.1083/jcb.202203021. Epub 2022 Apr 29.

Backbone

• Vector backbone: pmCherry-C1
• Modifications to backbone: Tag is dCherry-HA-FRB, dCherry is Dark mCherry, i.e. mCherry with a mutation (referred to as K70N although it is not at position 70) to render it non-fluorescent
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cacng2
• Species: M. musculus (mouse)
• Entrez Gene: Cacng2 (a.k.a. B230105C07Rik, B930041E13Rik, stargazer, stargazin, stg, wag, waggler)
• Promoter: CMV
• Tag / Fusion Protein:
  • dCherry-HA-FRB (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • Stargazin-darkmCherry-FRB.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 80406

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.23.441091v3.