FKBP-GFP-Sec61β
(Plasmid #172442)

• Purpose: Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag
• Depositing Lab: Stephen Royle
• Publication: Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213189. doi: 10.1083/jcb.202203021. Epub 2022 Apr 29.

Backbone

• Vector backbone: pFKBP-GFP-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Protein transport protein Sec61 subunit beta
• Species: H. sapiens (human)
• Promoter: CMV
• Tag / Fusion Protein:
  • FKBP-GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pEGFP-C2_FP
• 3′ sequencing primer: pEGFP-C2_RP

Resource Information

• Supplemental Documents:
  • FKBP-GFP-Sec61b.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 15108

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.23.441091v3.