pSF2C-A-RFP
(Plasmid #172432)

• Purpose: (Empty Backbone) StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)
• Depositing Labs: Paul Freemont, Simon Moore
• Publication: Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 10.1021/acssynbio.0c00581. Epub 2021 Jan 26.

Backbone

• Vector backbone: pAV-gapdh
• Backbone manufacturer: Jay Keasling lab
• Backbone size (bp): 4156
• Modifications to backbone: Removal of BsaI and BsmBI sites using Gibson DNA assembly and sequencing by primer walking (pSF1C-A-RFP). Golden Gate site from pTU2-A-RFP was sub-cloned via EcoRI and PscI.
• Vector type: Bacterial Expression, Synthetic Biology
• Tag / Fusion Protein:
  • None

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: VF2
• 3′ sequencing primer: VR

Resource Information

• Supplemental Documents:
  • pSF2C-A-RFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.11.16.384693v2 for bioRxiv preprint.