pSF1C-A-RFP
(Plasmid
#172431)
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Purpose(Empty Backbone) StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAV-gapdh
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Backbone manufacturerJay Keasling lab
- Backbone size (bp) 4156
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Modifications to backboneRemoval of BsaI and BsmBI sites using Gibson DNA assembly and sequencing by primer walking
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Vector typeBacterial Expression, Synthetic Biology
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Tag
/ Fusion Protein
- None
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer VF2
- 3′ sequencing primer VR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.16.384693v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSF1C-A-RFP was a gift from Paul Freemont & Simon Moore (Addgene plasmid # 172431 ; http://n2t.net/addgene:172431 ; RRID:Addgene_172431) -
For your References section:
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology. Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre C, de Los Santos EL, Freemont PS. ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 10.1021/acssynbio.0c00581. Epub 2021 Jan 26. 10.1021/acssynbio.0c00581 PubMed 33497199
Map uploaded by the depositor.
Map uploaded by the depositor.
