pBabe 3xFLAG mouse Bmf
(Plasmid #17243)

• Depositing Lab: Joan Brugge
• Publication: Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92.

Backbone

• Vector backbone: pBabe-neo
• Backbone manufacturer: Available from Addgene (#1767)
• Backbone size w/o insert (bp): 5330
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmf
• Alt name: Bcl2 modifying factor
• Species: M. musculus (mouse)
• Insert Size (bp): 550
• Entrez Gene: Bmf (a.k.a. AW260063, AW411872)
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

N-terminal FLAG epitopes were introduced through inclusion of corresponding sequence in the PCR primer.