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Addgene

MAC-CTSL
(Plasmid #172414)

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Item Catalog # Description Quantity Price (USD)
Plasmid 172414 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    MAC-tag-C
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Procathepsin L (Cathepsin L1)
  • Alt name
    CTSL
  • Species
    H. sapiens (human)
  • Entrez Gene
    CTSL (a.k.a. CATL, CTSL1, MEP)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • MAC-tag (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMVfor :CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer pCR3.1-BGHrev: TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC-CTSL was a gift from Markku Varjosalo (Addgene plasmid # 172414 ; http://n2t.net/addgene:172414 ; RRID:Addgene_172414)

  • For your References section:

    SARS-CoV-2-host proteome interactions for antiviral drug discovery. Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S, Vartiainen MK, Pietiainen V, Poso A, Varjosalo M. Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396. 10.15252/msb.202110396 PubMed 34709727
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