pMKO shRNA Bmf
(Plasmid
#17236)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMKO.1
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Backbone manufacturerAvailable at Addgene (#8452)
- Backbone size w/o insert (bp) 6700
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBmf shRNA
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Alt nameBmf
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SpeciesH. sapiens (human)
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Insert Size (bp)60
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Entrez GeneBMF
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
see article supplementary materials for shRNA seequence
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMKO shRNA Bmf was a gift from Joan Brugge (Addgene plasmid # 17236 ; http://n2t.net/addgene:17236 ; RRID:Addgene_17236) -
For your References section:
Functional role and oncogene-regulated expression of the BH3-only factor Bmf in mammary epithelial anoikis and morphogenesis. Schmelzle T, Mailleux AA, Overholtzer M, Carroll JS, Solimini NL, Lightcap ES, Veiby OP, Brugge JS. Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92. 10.1073/pnas.0700115104 PubMed 17360431