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pCDH_ss-SBP-EGFP
(Plasmid #172358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-hygro
  • Backbone manufacturer
    system biosciences
  • Backbone size w/o insert (bp) 8729
  • Total vector size (bp) 8783
  • Modifications to backbone
    pCDH was modified to remove the EF1 promoter and the puromycin resistance cassette driven by EF1 promoter. An IRES and a hygromycin resistance cassette were introduced downstream of SBP-EGFP.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER importing signal sequence
  • Alt name
    ss
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    54
  • Promoter CMV
  • Tag / Fusion Protein
    • SBP-EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It has to be used in combinaison with pCDH_Str-KDEL_neomycin (ID 65307) to mimic the conventional protein secretion

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH_ss-SBP-EGFP was a gift from Guido Kroemer (Addgene plasmid # 172358 ; http://n2t.net/addgene:172358 ; RRID:Addgene_172358)

  • For your References section:

    Identification of pharmacological inhibitors of conventional protein secretion. Zhao L, Liu P, Boncompain G, Loos F, Lachkar S, Bezu L, Chen G, Zhou H, Perez F, Kepp O, Kroemer G. Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.1038/s41598-018-33378-y. 10.1038/s41598-018-33378-y PubMed 30297865
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