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Addgene

pCMU-4
(Plasmid #172353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRISPomyces-2
  • Backbone size w/o insert (bp) 10994
  • Total vector size (bp) 10875
  • Modifications to backbone
    Removal of rpsL promoter for cas9. Replaced with D4, additional theophylline responsive riboswitch.
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D4-TheoR-cas9
  • Alt name
    Codon optimised cas9 behind D4 promoter and theophylline riboswitch
  • Alt name
    Cloned by Gibson Assembly
  • Species
    Synthetic
  • Insert Size (bp)
    4280
  • GenBank ID
  • Promoter D4

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACGATCCTCGCCCTGCT
  • 3′ sequencing primer GACGATGTTGCCGAAGAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMU-4 was a gift from Eriko Takano (Addgene plasmid # 172353 ; http://n2t.net/addgene:172353 ; RRID:Addgene_172353)

  • For your References section:

    Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces. Ye S, Enghiad B, Zhao H, Takano E. J Ind Microbiol Biotechnol. 2020 May;47(4-5):413-423. doi: 10.1007/s10295-020-02277-5. Epub 2020 May 4. 10.1007/s10295-020-02277-5 PubMed 32367443
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