pBAD-BRIC
(Plasmid
#172343)
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PurposeBioluminescent Red Indicator for Calcium (bacterial expression)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD/HisB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBRIC
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD-Forward
- 3′ sequencing primer pBAD-Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.12.06.471018v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-BRIC was a gift from Huiwang Ai (Addgene plasmid # 172343 ; http://n2t.net/addgene:172343 ; RRID:Addgene_172343) -
For your References section:
A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice. Tian X, Zhang Y, Li X, Xiong Y, Wu T, Ai HW. Nat Commun. 2022 Jul 8;13(1):3967. doi: 10.1038/s41467-022-31673-x. 10.1038/s41467-022-31673-x PubMed 35803917
