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pLV-REPORT(PGK/CMV)-GRE
(Plasmid #172336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 12867
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418), Hygromycin ; HSVtk

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    monomeric nuclear Tomato
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 3x SV40 NLS (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    d2eGFP
  • Species
    Synthetic
  • Promoter PGK/CMV
  • Tag / Fusion Protein
    • 3x SV40 NLS (N terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    HygR
  • Alt name
    hygromycin B phosphotransferase
  • Alt name
    hph

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-REPORT(PGK/CMV)-GRE was a gift from David Bersten (Addgene plasmid # 172336 ; http://n2t.net/addgene:172336 ; RRID:Addgene_172336)

  • For your References section:

    dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191
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