pLV-REPORT(PGK)-12xHRE
(Plasmid #172334)

• Purpose: hypoxic responsive reporter
• Depositing Lab: David Bersten
• Publication: Allen et al bioRxiv 2023.11.21.568191

Backbone

• Vector backbone: pLV
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 12806
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology
• Selectable markers: Neomycin (select with G418), Hygromycin ; HSVtk

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: monomeric nuclear Tomato
• Species: Synthetic
• Tag / Fusion Protein:
  • 3x SV40 NLS (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: d2eGFP
• Species: Synthetic
• Promoter: PGK
• Tag / Fusion Protein:
  • 3x SV40 NLS (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: HygR
• Alt name: hygromycin B phosphotransferase
• Alt name: hph

Resource Information

• Supplemental Documents:
  • pLV-REPORT (PGK) 12x HRE.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.