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Addgene

pBAD-MtrxRFP2
(Plasmid #172323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisB
  • Total vector size (bp) 5273
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
  • Alt name
    MtrxRFP2
  • Species
    Synthetic
  • Insert Size (bp)
    1317
  • Promoter pBAD
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-MtrxRFP2 was a gift from Huiwang Ai (Addgene plasmid # 172323 ; http://n2t.net/addgene:172323 ; RRID:Addgene_172323)

  • For your References section:

    Improved Red Fluorescent Redox Indicators for Monitoring Cytosolic and Mitochondrial Thioredoxin Redox Dynamics. Pang Y, Zhang H, Ai HW. Biochemistry. 2022 Mar 1;61(5):377-384. doi: 10.1021/acs.biochem.1c00634. Epub 2022 Feb 8. 10.1021/acs.biochem.1c00634 PubMed 35133140
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