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Arabidopsis thaliana Atm3 pET21a+
(Plasmid #172321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone size w/o insert (bp) 5340
  • Total vector size (bp) 7560
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arabidopsis thaliana Atm3
  • Alt name
    AtAtm3
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2220
  • GenBank ID
    NM_125212.3
  • Entrez Gene
    ABCB25 (a.k.a. AT5G58270, ABC transporter of the mitochondrion 3, ARABIDOPSIS THALIANA ABC TRANSPORTER OF THE MITOCHONDRION 3, ATATM3, ATM3, ATP-binding cassette B25, HALF-MOLECULE ABC TRANSPORTER ATM3, MCK7.14, MCK7_14, STA1, STARIK 1)
  • Tag / Fusion Protein
    • 6xHis tag (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ordered from Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Arabidopsis thaliana Atm3 pET21a+ was a gift from Douglas Rees (Addgene plasmid # 172321 ; http://n2t.net/addgene:172321 ; RRID:Addgene_172321)

  • For your References section:

    Glutathione binding to the plant AtAtm3 transporter and implications for the conformational coupling of ABC transporters. Fan C, Rees DC. Elife. 2022 Mar 25;11. pii: 76140. doi: 10.7554/eLife.76140. 10.7554/eLife.76140 PubMed 35333177
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