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pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
(Plasmid #172318)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEG302
  • Backbone size w/o insert (bp) 7570
  • Total vector size (bp) 27502
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
  • Alt name
    SunTag-MQ1v
  • Species
    A. thaliana (mustard weed); Mollicutes Spiroplasma
  • Insert Size (bp)
    19930

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (unknown if destroyed)
  • 3′ cloning site BsiWI (unknown if destroyed)
  • 5′ sequencing primer GGCAGACAAACAAAAGAATGG
  • 3′ sequencing primer GTGGTGGTTTCACCTTTCAGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18 was a gift from Steven Jacobsen (Addgene plasmid # 172318 ; http://n2t.net/addgene:172318 ; RRID:Addgene_172318)

  • For your References section:

    CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by a bacterial CG-specific DNA methyltransferase. Ghoshal B, Picard CL, Vong B, Feng S, Jacobsen SE. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 10.1073/pnas.2125016118. 10.1073/pnas.2125016118 PubMed 34074795
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