pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
(Plasmid #172317)

• Purpose: CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs
• Depositing Lab: Steven Jacobsen
• Publication: Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 10.1073/pnas.2125016118.

Backbone

• Vector backbone: pEG302
• Backbone size w/o insert (bp): 15826
• Total vector size (bp): 21241
• Vector type: Plant Expression, CRISPR, Synthetic Biology
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
• Alt name: dMQ1
• Species: Mollicutes Spiroplasma
• Insert Size (bp): 13669

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: ATTTCTATCTAGATCTGGTGTT
• 3′ sequencing primer: TAAGGATCTGAGCTACACATGCTCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry