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Addgene

pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
(Plasmid #172316)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172316 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEG302
  • Backbone size w/o insert (bp) 15826
  • Total vector size (bp) 21241
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
  • Alt name
    MQ1v
  • Species
    Mollicutes Spiroplasma
  • Insert Size (bp)
    13669

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site AscI (destroyed during cloning)
  • 5′ sequencing primer ATTTCTATCTAGATCTGGTGTT
  • 3′ sequencing primer TAAGGATCTGAGCTACACATGCTCA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18 was a gift from Steven Jacobsen (Addgene plasmid # 172316 ; http://n2t.net/addgene:172316 ; RRID:Addgene_172316)
  • For your References section:

    CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by a bacterial CG-specific DNA methyltransferase. Ghoshal B, Picard CL, Vong B, Feng S, Jacobsen SE. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 10.1073/pnas.2125016118. 10.1073/pnas.2125016118 PubMed 34074795