NLS-4LNDDE488Q-pCDNA3.1(+)
(Plasmid #172314)

• Purpose: Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
• Depositing Lab: Joshua Rosenthal
• Publication: Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi: 10.1080/15476286.2017.1387711. Epub 2017 Nov 13.

Backbone

• Vector backbone: pcDNA3.1(+)
• Total vector size (bp): 7175
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lambda N peptide - human ADAR2 Deaminase Domain E488Q
• Species: H. sapiens (human); Escherichia virus Lambda
• Insert Size (bp): 1752
• Mutation: changed the Glutamic acid 488 in the Deaminase Domain for Glutamine
• Promoter: CMV
• Tags / Fusion Proteins:
  • Nuclear Localization Signal of SV40 (simian virus 40) large T antigen (N terminal on insert)
  • Flag (N terminal on backbone)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • NLS-4LNDDE488Q-pCDNA3.1(+)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry