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Addgene

pAAV-Sst22-GFP
(Plasmid #172312)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172312 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-mDlx-GFP-Fishell-1
  • Backbone manufacturer
    Addgene # 83900
  • Backbone size w/o insert (bp) 4954
  • Total vector size (bp) 5674
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sst22
  • Alt name
    GRE22
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    720
  • Promoter pB-Globin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer tcactaggggttcctgcgg
  • 3′ sequencing primer tgggcataaaagtcagggcaga
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Sst22-GFP was a gift from Michael Greenberg (Addgene plasmid # 172312 ; http://n2t.net/addgene:172312 ; RRID:Addgene_172312)
  • For your References section:

    A scalable platform for the development of cell-type-specific viral drivers. Hrvatin S, Tzeng CP, Nagy MA, Stroud H, Koutsioumpa C, Wilcox OF, Assad EG, Green J, Harvey CD, Griffith EC, Greenberg ME. Elife. 2019 Sep 23;8. pii: 48089. doi: 10.7554/eLife.48089. 10.7554/eLife.48089 PubMed 31545165