pIVTR(T7A)-ATOH1 (SA Substitutions)
(Plasmid
#172309)
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PurposeIn vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172309 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIVTR
- Backbone size w/o insert (bp) 2074
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATOH1
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SpeciesH. sapiens (human)
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Insert Size (bp)1065
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MutationS331A, S342A
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Entrez GeneATOH1 (a.k.a. ATH1, DFNA89, HATH1, MATH-1, bHLHa14)
- Promoter T7 promoter A-initiating
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVTR(T7A)-ATOH1 (SA Substitutions) was a gift from Patrik Verstreken (Addgene plasmid # 172309 ; http://n2t.net/addgene:172309 ; RRID:Addgene_172309)