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pQE9-His-p97deltaD2(K251A)
(Plasmid #17230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE9
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    needs lacIq, e.g. XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p97
  • Alt name
    VCP, valosin-containing protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2415
  • Mutation
    change codon 457 to stop codon, change lysine 251 to alanine
  • Entrez Gene
    Vcp (a.k.a. 3110001E05, CDC48, p97, p97/VCP)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE9-His-p97deltaD2(K251A) was a gift from Hemmo Meyer (Addgene plasmid # 17230 ; http://n2t.net/addgene:17230 ; RRID:Addgene_17230)

  • For your References section:

    Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin. Ramadan K, Bruderer R, Spiga FM, Popp O, Baur T, Gotta M, Meyer HH. Nature. 2007 Dec 20. 450(7173):1258-62. 10.1038/nature06388 PubMed 18097415
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