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Addgene

pIVTR(T7A)-Cas9n-RT-T2A-PuroR
(Plasmid #172296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVTR
  • Backbone size w/o insert (bp) 2074
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9n-RT-T2A-PuroR
  • Species
    Other
  • Insert Size (bp)
    7027
  • Mutation
    H840A (Cas9)
  • Promoter T7 promoter A-initiating

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVTR(T7A)-Cas9n-RT-T2A-PuroR was a gift from Patrik Verstreken (Addgene plasmid # 172296 ; http://n2t.net/addgene:172296 ; RRID:Addgene_172296)
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